Information required by an autoclave specialist!
Different autoclave components
| chamber | Electrical panel | Air care unit |
| chamber | Steam gauge | Steam regulator |
| Doors | Pressure sensors | Reassurance valve |
| Heat exchanger | Solenoid valves | One-way valve |
| Control system | Pneumatic valves | microswitch |
| Data transfer port | Pneumatic motor | Control panel |
| chamber | Electrical panel | Air care unit |
| Recorder | Pneumatic jacks | Vent air filter |
| Temperature gauges | Door washer | Steam filter |
| Temperature sensors | traps | water filter |
| Temperature transmitters | Filters | Manual tap |
Autoclave installation conditions and prerequisites:
| .Location based on actual dimensions and size in LAYOUT installation location |
| .The technical space on each side should be at least one meter |
| .The height of the installation site should be such that there is space up to one meter above the autoclave |
| .Have an installation location in the technical air conditioning |
| .Consider a hot sewer line |
| .The floor should be considered at the installation site |
| .Compressed air should be considered |
| .Soft water should be considered |
| .Cold or chilled water should be considered |
| Open the door |
| .Industrial steam should be considered |
| .Clean steam should be considered |
| .Consider the steam return line |
| .Filter chamber output |
| .Proper electricity should be considered |
| .Lighting should be considered in the technical part |
| .Clean and non-clean parts should be considered during installation |
| .Open the service side to the unclean |
| .The floor of the installation site should be waterproofed |
| .Join the floor of the installation site with epoxy |
| Leave the junction of the wall and the wall up to a height of 10 to 15 cm from the floor of the waterproofing .and seal |
| .Do not connect the condensate outlet to the positive pressure line and discharge by gravity |
Tasks of autoclave operator:
| Internal inspection of autoclave (status of sensors – filters – drain – doors, etc.) |
| plete the preparation checklist |
| Installation preparation (steam – compressed air – water – main electricity) |
| Turn on the autoclave |
| Program selection (solids – liquids and) .. |
| Adjust parameters (sterile temperature – sterilization time – drying time – number of initial and final vacuum pulses, etc.) |
| Loading (picking up items or containers containing materials on trolleys or floors – placing sensors inside a sample container and Proper transfer of the trolley to the chamber and close the door |
| Start the selected program |
| Monitoring |
| Open the door |
| .Remove the material trolley and sterilized lotus so that the sensors are not damaged |
| Complete the logbook |
| Close the autoclave door |
| Complete the autocla completion checklist |
| (Periodic cleaning (when the autoclave has cooled |
| Proper use of off-label tags (unusable) |
| Request engineering services |
| Carrying out preventive maintenance activities and recording records |
If you are a technician in the field of laboratory equipment repair and hospital equipment repair, you should have high technical knowledge in the field of devices. In this article, we will talk about what an autoclave technician should know.
| Knowledge of sterilization | Understanding the sterile mechanism of autoclave doors | Understand the structure and system of autoclave |
| Understand how it works | Understand the components and their role | Autoclave Operator |
| Documents (service and maintenance documents, preventive maintenance program, qualification, etc.) | Familiarity with the principles of safety of working with autoclave | Preventive Maintenance |
| Requirements and technical specifications for selection and purchase | Competency | Validation |
| Installation costs and its quantitative and qualitative specifications | Autoclave troubleshooting and repair | Installation method |
Different sterilization methods
| Sterile with hot air like sterile glass vials in Furia |
| Sterile with gases (ethylene oxide, hydrogen peroxide, ozone) |
| …sterile with gamma rays and uv rays such as sterile plastic objects and surfaces and |
| … Sterile with filtration (0.2µ) such as sterilizing the final product and |
| …Steam sterilized (autoclave) such as dishes, liquids, clothes and |
Steam sterilization and its applications
| In research centers and production of biological products |
| In hospitals and clinics |
| In the production of biological products (vaccines) |
| In biological research and disease control centers |
| In the process of food production |
| In the production centers of medical and hospital equipment and supplies |
| In order to reduce the population of pathogenic and harmful microorganisms, sterile operations are performed with .appropriate methods and equipment |
| The goal is to sterilize the microbial population by 90%. |
Autoclave and steam sterilization mechanism
| Sterilization is the basis of autoclave sterilization by transferring heat energy to the microbial agent and increasing its temperature. |
| As the microbial mass temperature rises and the kinetic energy increases, the molecular bond of hydrogen in the structure of proteins disintegrates. |
| Steam transmits energy to microorganisms. |
| Water molecules with energy in contact with a microbial mass at a lower temperature transfer their energy to a microbial mass and eventually condense. |
| The heat capacity of steam is high. |
| Advantage of steam sterilization: efficiency – speed – reliability |
| Steam conditions and characteristics for sterilization: dryness, suitable temperature, suitable pressure |
| An autoclave is a device for creating suitable conditions for steam sterilization |